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Table 2 The sequence of primers, RNA oligo in this study

From: Upregulation of miR-330-5p is associated with carotid plaque’s stability by targeting Talin-1 in symptomatic carotid stenosis patients

 

Sequence (5′ to 3′)

Primers

 hsa-miR-6721-5p

Forward: GATTGGGCAGGGGCTTATTG

Reverse: CAGTGCAGGGTCCGAGGTAT

 hsa-miR-20a-3p

Forward: CGGAGACTGCATTATGAGCAC

Reverse: CAGTGCAGGGTCCGAGGTAT

 hsa-miR-330-5p

Forward: GCCTCTCTGGGCCTGTGTC

Reverse: CAGTGCAGGGTCCGAGGTAT

 hsa-miR-326

Forward: CATCGCCTCTGAGCCCTTC

Reverse: CAGTGCAGGGTCCGAGGTAT

 hsa-miR-4527

Forward: AGGTGGTCTGCAAAGAGATGA

Reverse: CAGTGCAGGGTCCGAGGTAT

 hsa-miR-6503-5p

Forward: GCGAGGTCTGCATTCAAATCC

Reverse: CAGTGCAGGGTCCGAGGTAT

 hsa-miR-432-5p

Forward: GGCTCTTGGAGTAGGTCATTGG

Reverse: CAGTGCAGGGTCCGAGGTAT

 hsa-miR-7150

Forward: GGTAGACTGGCAGGGGGAG

Reverse: CAGTGCAGGGTCCGAGGTAT

 hsa-miR-4260

Forward: GCAGACTTGGGGCATGGA

Reverse: CAGTGCAGGGTCCGAGGTAT

 U6B

Forward: CGCTTCGGCAGCACATATAC

Reverse: AAAATATGGAACGCTTCACGA

RNA oligo

 miR-330-5p mimics

AAGCUGCCAGUUGAAGAACUGU

ACAGUUCUUCAACUGGCAGCUU

 miR-330-5p mimics NC

GCAACCUGUUAGACUAUGGAGA

UCUCCAUAGUCUAACAGGUUGC

 Talin-1

GUCCAUCAUUCAUGCGAAATT

UUUCGCAUGAAUGAUGGACTT

 Negative control

UUCUCCGAACGUGUCACGUTT

ACGUGACACGUUCGGAGAATT

 GAPDH Positive control

UGACCUCAACUACAUGGUUTT

AACCAUGUAGUUGAGGUCATT

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BMC Cardiovascular Disorders

ISSN: 1471-2261

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