Fig. 6

a Protein expression levels of total SHP2, phosphorylated SHP2, total ERK and phosphorylated ERK in VSMCs. VSMCs were treated with PHPS1 or DMSO for 30 min and then with 100 μg/ml oxLDL for 10 min. Data are presented as the mean ± SE. *p < 0.05 vs. the oxLDL group. b Effects of SHP2 on VSMC proliferation. After preincubation in the presence or absence of 10 μM PHPS1 or an equal volume of DMSO for 30 min at 37 °C, cells were stimulated with oxLDL (100 μg/mL) for 24 h. BrdU (1 μmol/L) was added, and the cells were incubated for 3 h. BrdU incorporation was quantified using the BrdU cell proliferation assay kit. The percentage of BrdU-positive VSMCs was determined in the three groups. Compared with the other groups, the PHPS1 treatment group showed a decrease in the percentage of BrdU-positive VSMCs. *p < 0.05 vs. the oxLDL group