Fig. 1
Physiological and pharmacological AngII results in significantly different Ca2+ signaling pattern. a Representative [Ca2+]i traces from Fura Red loaded HEK cells treated with repeated 1 nM (left) or 100 nM (right) AngII for two minutes at times indicated by the arrows, with 5 min recovery after the first exposure and 30 min after second exposure. b Quantification of peak [Ca2+]i response to repeated AngII treatment 1 nM (open bars) and 100 nM (grey bars) as shown in A. Responses are calculated as peak signal above baseline. *: p ≤ 0.05 compared to 1st AngII exposure, **: p ≤ 0.01 compared to 1st AngII exposure. n = 4 - 8 experiments. c The cartoon shows the principle used to detect AT1R in plasma membrane. Signal from Anti-HA-Alexa594 antibody show plasma membrane AT1R, Venus signal show the total abundance of AT1R. Bars show relative change of plasma membrane AT1R abundance 3 min and 30 min after exposure to AngII, 1 nM AngII open bars and 100 nM grey bars. Data is normalized to untreated control membrane fraction. n = 5 experiments