Table 2 Primer sequences used for real time fluorescent quantitative polymerase chain reaction
From: miR-21 enhances cardiac fibrotic remodeling and fibroblast proliferation via CADM1/STAT3 pathway
Target mRNA | Primer | Sequence |
|---|---|---|
Rat CADM1 | Forward | 5'-TGGCGTTACCTT GGTAACC-3' |
Reverse | 5'-GGTGTTGAGCCCTTTCCAG-3' | |
Rat α-SMA | Forward | 5' TGGCCACTGCTGCTTCCTCTTCTT 3' |
Reverse | 5' GGGGCCAGCTTCGTCATACTCCT 3' | |
Rat STAT3 | Forward | 5'-CACCCATAGTGAGCCCTTGGA-3' |
Reverse | 5'-TGAGTGCAGTGACCAGGACAGA-3' | |
Rat β-actin | Forward | 5' TGG AATCCTGTGGCATCCATGAAAC 3' |
Reverse | 5' ACGCAGCTCAGT AACAGTCCG 3' | |
Human CADM1 | Forward | 5'-TCAACACGCCGTACTGTCTG -3' |
Reverse | 5'-GTGGGAGGAGGGATAGTTGTG -3' | |
Human STAT3 | Forward | 5'- GAAGAATCCAACAACGGCAG-3' |
Reverse | 5'- TCACAATCAGGGAAGCATCAC-3' | |
Human β-actin | Forward | 5'-GCTCGTCGTCGACAACGGCTC-3' |
Reverse | 5'-CAAACATGATCTGGGTCATCTTCTC-3' |