Fig. 4

Knockdown of miR-21 inhibits the cardiac fibroblasts proliferation. a One Step Real-time PCR showed that transfection miR-21 inhibitor decreased the expression of miR-21 in cardiac fibroblasts. miR-21 inhibitor group is decreased significantly compare with vehicle group. b Cell viability was determined using MTT assay. 3 × 10⁵ cardiac fibroblasts cells were seeded in 96-well plates and transfected with 60 nM miR-21 inhibitor (24 h, 48 h). Knockdown of miR-21 caused a significant inhibition of cell activation and proliferation in TGF-β1-treated cardiac fibroblasts and the results were directly tested by MTT assay. c Cardiac fibroblasts cells were transfected with 60 nM miR-21 inhibitor for 48 h. Then cells were harvested and the cell-cycle distribution was analyzed by Flow cytometry analysis. Data are representative of at least three separate experiments. *P < 0.05, **P < 0.01 versus Vehicle or Control; ^# P < 0.05 versus model