Fig. 2

The time-course expression of sema 3A in myocardial infarction (MI) models after lentivirus-sema 3A-shRNA treatment. a, b Western blotting was conducted to detect the effects of lentivirus-sema 3A-shRNA on the protein level of sema 3A (95 KDa) at various time points (1, 2, and 5 weeks). The relative expression of sema 3A was normalized to GAPDH (36 KDa). c Relative mRNA expression of sema 3A was detected using real-time quantitative RT-PCR. The relative gene expression of sema 3A was analyzed using the 2^-ΔΔCT method. Data are presented as means ± SD. *P < 0.05 vs. sham group. †P < 0.05 vs. MI-siRNA group