Table 1 The primers of cDNA of ion channels related genes and GAPDH/Gapdh for RT-PCR analysis
Gene | F | R |
|---|---|---|
SCN3B(human) | attgtttcccctggcttctc | gcctccacctcctctctctt |
Scn3b(mouse) | catcctcctggtcttcctcac | cgggtaccacagagttctcct |
P53(human) | ccccagccaaagaagaaacc | gcctgggcatccttgagttc |
CACNA1C | ggctgctgaggattttcaag | acacagtgaggagggactgg |
KCNE3 | tccagagacatcctgaagagg | ggtctccgttccattggtag |
KCND3 | tgatgttttatgccgagaagg | ccatggtgactccagctctt |
KCNN1 | agccaccctctctcccagtca | aggggttgggctcgctgca |
SCN2A | atgatgaaaatggcccaaag | ggtggcactgaatcgagaga |
SCN3A | atgctgggctttgttatgct | ttgctcctttcccagtaagc |
SCN4A | tccagcagggttggaatatc | tgccaatgatcttgatgagc |
SCN5A | ccagatctctatggcaatcca | gaatcttcacagccgctctc |
SCN9A | gatgatgaagaagccccaaa | gtggcattgaaacggaagat |
SCN10A | acttgaaagcctgcaaccag | cactaaaccgggaaatggtc |
SCN1B | tctaccgcctgctcttcttc | ggcagcgatcttcttgtagc |
SCN2B | atccatctgcaggtcctcat | catctgtgctcagcttctgc |
KCNQ1 | ggccacggggactctcttc | tccgtcccgaagaacacca |
KCNA5 | ggccgaccccttcttcatc | gcagctcgaaggtgaaccag |
KCNH2 | gaggagcgcaaagtggaaatc | gccccatcctcgttcttcac |
KCNJ5 | ttctgaagggagcaggtcat | cctagaatcgccagccatag |
KCNE2 | cttgtgtgcaacccagaaga | gtcttccagcgtctgtgtga |
Gapdh(mouse) | tggccttccgtgttcctacc | ggtcctcagtgtagcccaagatg |
GAPDH(human) | aaggtgaaggtcggagtcaac | ggggtcattgatggcaacaata |