Fig. 5

The protection effects of FGF21 on apoptosis were through suppress CHOP signaling in macrophages. a The knockdown efficiency of CHOP was tested by western blotting in the THP1 derived macrophages transfected by siRNA. DR5 mRNA level was measured by Real-time PCR in THP1 derived macrophages with si-CHOP transfection treated by ox-LDL (b) or 7-KC (C) in the presence or absence of FGF21. 18S rRNA was used as internal control. d-e Quantification of apoptosis cells in FACS assay of THP1 cells treated as indicated. Data are presented as the mean ± S.E. of at least three independent experiments. *, p < 0.05, **, p < 0.01. N.S, no significance