Fig. 3

The effect of FGF21 on macrophages is ERK signaling pathway independent. a The knockdown efficiency of ERK1/2 was tested by western blotting in the THP1 derived macrophages transfected by siRNA. c Phosphorylated ERK1/2 level was tested in THP1 derived macrophages with or without PD98059 treatment for 24 h. The intracellular total cholesterol (TC) content was measured in the THP1 derived macrophages treated by ox-LDL in the presence or absence of FGF21 with ERK1/2 knockdown b or ERK1/2 specific inhibitor PD98059 d. Data are presented as the mean ± S. e. of at least four independent experiments. *, p < 0.05, **, p < 0.01. N.S, no significance