Figure 3

PAI-1 expression levels in response to TNFα, TGFβ, or insulin in WRN knocked down fibroblasts. Five synchronized heterozygous fibroblasts cultures (1367CR/1074LF) were transfected with a molecule of iRNA against WRN messenger (B), or with a scrambled RNA as control (A). PAI-1 inducers (TNFα, TGFβ, or insulin) were added 24 hours post-transfection and PAI-1 and WRN expression was evaluated by semiquantitative RT-PCR 24 hours after agonist challenge. Normalized values of mRNA were grouped as mean ± standard error of arbitrary units. ANOVA was used to test differences across treatments (control, scrambled and iRNA). * Significant differences compared with scrambled by Tukey test; P < 0.05 and ** P < 0.01.