Figure 5
From: Estrogen-induced DNA synthesis in vascular endothelial cells is mediated by ROS signaling
Antioxidant N-acetylcysteine blocks estrogen-induced DNA synthesis. Human umbilical vein endothelial cells pretreated with the antioxidant N-acetylcysteine (NAC) showed a significant reduction in E2-induced DNA synthesis. Data from three independent experiments are presented as ROS production with controls set at 100% (± SD). Values that are significantly different from E2 treatment alone (P < 0.05) are indicated with an asterisk (*). Significant increases in DNA synthesis by E2 (P < 0.05) are indicated by (#).