Table 7 TGF-β1 regulated gene expression of ALK1 response genes in HMEC-1 cells. HMEC-1 cells (human microvascular endothelial cell line) were incubated with the indicated amounts of TGF-β1. Gene expression after 24 hours was assesed by qRT-PCR. Gene expression profile of non-TGF-β1 treated cells served as reference. qRT-PCR based relative gene expression was calculated using the comparative ΔΔC_T method. RQ represents the relative expression level (fold change) compared to the reference sample. RQ_Max/Min are the relative maximum/minimum expression levels of the mean expression level RQ. The right column shows in comparison the average RQ level of three independent experiments with HMEC-1 cells infected with AdALK1^QD (see Table 4). Genes with RQ values in bold are considered to be up-regulated and RQ values in italic are considered to be down-regulated.

0.64 (1.55/0.27)

0.56 (0.74/0.43)

4.55

0.17 (0.32/0.09)

0.14 (0.16/0.13)

1056.07

0.74 (0.83/0.66)

0.78 (0.87/0.69)

14.56

1.75 (2.00/1.53)

2.65 (3.51/2.01)

2.28

3.26 (3.50/3.05)

3.37 (3.99/2.85)

3.93

0.80 (1.05/0.61)

0.96 (1.07/0.87)

3.98

3.27 (3.47/3.09)

3.06 (3.52/2.66)

3.87

0.89 (1.21/0.65)

1.32 (2.41/0.72)

4.29

0.94 (1.12/0.79)

1.14 (1.62/0.80)

2.08

0.72 (0.79/0.65)

0.91 (1.07/0.78)

6.56

2.44 (2.72/2.19)

2.60 (2.82/2.39)

4.26

1.07 (1.16/0.98)

1.43 (1.64/1.25)

1.36

0.97 (1.10/0.85)

0.99 (1.04/0.94)

1.26

1.04 (1.22/0.88)

1.22 (1.29/1.16)

2.36

1.32 (1.71/1.02)

1.47 (1.88/1.16)

1.45

1.01 (1.23/0.83)

1.23 (1.45/1.04)

1.40

1.26

1.01

5079.92*