Skip to main content

Table 7 TGF-β1 regulated gene expression of ALK1 response genes in HMEC-1 cells. HMEC-1 cells (human microvascular endothelial cell line) were incubated with the indicated amounts of TGF-β1. Gene expression after 24 hours was assesed by qRT-PCR. Gene expression profile of non-TGF-β1 treated cells served as reference. qRT-PCR based relative gene expression was calculated using the comparative ΔΔCT method. RQ represents the relative expression level (fold change) compared to the reference sample. RQMax/Min are the relative maximum/minimum expression levels of the mean expression level RQ. The right column shows in comparison the average RQ level of three independent experiments with HMEC-1 cells infected with AdALK1QD (see Table 4). Genes with RQ values in bold are considered to be up-regulated and RQ values in italic are considered to be down-regulated.

From: ALK1 signalling analysis identifies angiogenesis related genes and reveals disparity between TGF-β and constitutively active receptor induced gene expression

  HMEC-1
  0.5 ng TGF-β1/ml 4 ng TGF-β1/ml AdALK1QD
Gene [GenBank number] 24 hours RQ (RQMax/Min) 24 hours RQ (RQMax/Min) RQ
GADD153 [S40706] 0.64 (1.55/0.27) 0.56 (0.74/0.43) 4.55
IL-8 [M28130] 0.17 (0.32/0.09) 0.14 (0.16/0.13) 1056.07
SMAD6 [U59914] 0.74 (0.83/0.66) 0.78 (0.87/0.69) 14.56
COL5A1 [D90279] 1.75 (2.00/1.53) 2.65 (3.51/2.01) 2.28
HEF1 [L43821] 3.26 (3.50/3.05) 3.37 (3.99/2.85) 3.93
TLR4 [U93091] 0.80 (1.05/0.61) 0.96 (1.07/0.87) 3.98
ET-1 [J05008] 3.27 (3.47/3.09) 3.06 (3.52/2.66) 3.87
CARP [X83703] 0.89 (1.21/0.65) 1.32 (2.41/0.72) 4.29
HPTPη [D37781] 0.94 (1.12/0.79) 1.14 (1.62/0.80) 2.08
ID1 [S78825] 0.72 (0.79/0.65) 0.91 (1.07/0.78) 6.56
BMP6 [M60315] 2.44 (2.72/2.19) 2.60 (2.82/2.39) 4.26
ALK5 [L11695] 1.07 (1.16/0.98) 1.43 (1.64/1.25) 1.36
TβRII [M85079] 0.97 (1.10/0.85) 0.99 (1.04/0.94) 1.26
Endoglin [J05481] 1.04 (1.22/0.88) 1.22 (1.29/1.16) 2.36
c-myc [V00568] 1.32 (1.71/1.02) 1.47 (1.88/1.16) 1.45
ALK1 [L17075] ABI assay 1.01 (1.23/0.83) 1.23 (1.45/1.04) 1.40
ALK1 [L17075] own assay 1.26 1.01 5079.92*
  1. *, ectopic expression