Table 2 List of genes analysed by qRT-PCR and the corresponding pre-designed gene-specific primer pair assay (ABI Assay-on-Demand™, Applied Biosystems). Where possible RNA specific assays were selected which are depicted by _m1 suffix. For CHOP _g1 suffix signifies that the assay may detect DNA and for ID1 the _s1 suffix indicates both the primers and probe are in a single exon.
GENE | ABI Assays-on-Demand™ |
|---|---|
ALK1/ACVRL1 | Hs00163543_m1 |
ALK5/TGFBR1 | Hs0061319_m1 |
BMP6 | Hs00233470_m1 |
CARP | Hs00173317_m1 |
CD148/HPTPη | Hs00174561_m1 |
CHOP/DDIT3/GADD153 | Hs00358796_g1 |
C-MYC | Hs00153408_m1 |
Collagen VαI/COL5A1 | Hs00609088_m1 |
Endoglin/ENG | Hs00164438_m1 |
Endothelin/END1 | Hs00174961_m1 |
HEFI/NEDD9 | Hs00610590_m1 |
ID1 | Hs00704053_s1 |
IL-8 | Hs00174103_m1 |
SMAD6/MADH6 | Hs00178579_m1 |
TGFβR2 | Hs00559661_m1 |
TLR4 | Hs00152939_m1 |
GAPDH | Hs99999905_m1 |