Figure 3
Confocal microscopic examination of titin disruption in cell culture and myocardial tissue. (a) Confocal microscopy showing disruption of sarcomeric structure stained for titin in HL-1 cell culture when exposed to iron (III) chloride (FeCl3) for 72 hours. (b) Confocal microscopy showing disorganized pattern of titin stain with loss of the striated pattern in ventricular myocardial tissue of a mouse overloaded with iron and the normal striations seen in a control mouse (upper panel). The curves in the middle panel represent the fluorescent signal intensity along the yellow lines as shown in the lower panel.