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Table 1 Sequence of primers used in real-time RT-PCR

From: Proprotein convertase subtilisin/kexin type 9 expression is transiently up-regulated in the acute period of myocardial infarction in rat

Gene

Forward primer (5′ → 3′)

Reverse Primer (5′ → 3′)

PCSK9

CGGGAAGGACATCATCGG

GGTTCAGCATCATAGCCACAAT

LDLR

GATTGGCTATGAGTGCCTATGTC

GTGAAGAGCAGAAACCCTATGG

SREBP2

AGCATACCGCAAGGTGTTCC

CCAGGTGTCTACTTCTCCGTGT

HNF1α

ATGACACGGATGACGATGGG

ATGGGTCCTCCTGAAGAAGTGA

GAPDH

ACAGCAACAGGGTGGTGGAC

TTTGAGGGTGCAGCGAACTT

  1. PCSK9 = proprotein convertases subtilisin kexin 9; LDLR = low-density lipoprotein receptor; SREBP-2 = sterol response element binding protein-2; HNF1α = hepatocytenuclear factor 1α; GAPDH = glyceraldehyde-3-phosphate dehydrogenase.
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BMC Cardiovascular Disorders

ISSN: 1471-2261

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