Figure 4

LV myocardial activity levels of enzymes implicated in fatty acid β-oxidation, glucose metabolism and mitochondrial energy production in 9-month AR rats and relative evolution over time. HADH (hydroxyacyl-Coenzyme A dehydrogenase; A), PFK (phosphofructokinase; B), citrate synthase (CS; C) enzymatic activities were measured in LV homogenates from at least 10 animals in each group as described in the Methods. Hexokinase (HK; D), complex 1 (ETC. complex 1, rotenone-sensitive activity; E) and LDH (lactate dehydrogenase (F) activities were measured in LV homogenates from 10 270-day animals. Results are reported relative to activity level measured in 90-day sham rats (A, B and C) or in μmoles/min/mg of tissue (D, E, and F) or. *p < 0.05, **p < 0.01 and ***p < 0.001 between sham and AR groups.