Figure 7

The high fat diet in AR rats seems to impair the switch from fatty acid to glucose as preferred myocardium energy substrate. HK (A; hexokinase) and PFK (B; phosphofructokinase) enzymatic activities were measured as described in the Materials and Methods. Evaluation by real-time quantitative RT-PCR of the LV mRNA levels of other genes related to glucose oxidation is illustrated in panel C. D, Increased Glut4 translocation to the plasma membrane is reversed by the high fat diet in AR rats as evaluated by immunoblotting. E, Recruitment of IRS1 to the membrane in the LVs of AR rats is partly reversed by the high fat diet. F, Representative blots are illustrated. *: p < 0.05 and ***: p < 0.001 between sham and AR groups. §: p < 0.05, §§: p < 0.01 and §§§: p < 0.001 vs. corresponding sham or AR group.