The high fat diet impact on LV signaling in AR rats. Phosphorylated protein content of various signaling molecules implicated in cardiac growth and hypertrophy were evaluated by immunoblotting. SC protein levels were normalized to 1 and are represented as a black line on the different graphs (A and C). *: p < 0.05, **: p < 0.01 and ***: p < 0.001 between sham and AR groups. §: p < 0.05 vs. corresponding group on control diet. ARC animals are represented by the white columns and the ARHF the solid ones. Representative blots are also illustrated (B and D).