Figure 2

The representative immunohistochemical photomicrographs of Hsp90/p-Akt (Thy308). The positive expression of Hsp90 was indicated by brown staining (original magnification × 400). Representative immunohisto- chemical data depicting cardiac Hsp90/p-Akt and cleaved caspase-3 expression in the LPS-treated mice (×400). Panels A and B, Representative pictures reveal a significant decrease in Hsp90 (A) and p-Akt (B) positive expression in the cytoplasm of the myocytes after LPS treatment. Panel C, Representative pictures of cleaved caspase-3 (the antibody was specific to detect the large fragment 17/19 KDa of activated caspase-3) expression in the cytoplasm after LPS treatment or LPS plus calpain inhibitors, calpain inhibitor III and PD150606 in the septic mice. Panel D: Representative quantitative data of Hsp90/p-Akt and cleaved caspase-3 expression in the septic mice and after treatment with calpain inhibitors. The values were from ten high power fields each section randomly selected and counted manually. CI, calpain inhibitor-III; PD, PD150606. * P < 0.05 vs. sham,# P < 0.05 vs. LPS.