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Figure 4 | BMC Cardiovascular Disorders

Figure 4

From: Loss of ectonucleotidases from the coronary vascular bed after ischemia-reperfusion in isolated rat heart

Figure 4

Ectonucleotidase activity in the effluents of immediately after reperfusion. (A) Effluents collected as indicated in Figure 3 were incubated with 100 μM ATP, AMP or adenosine (Ado) for 10 or 30 min, and the decrease in each substrate was measured by HPLC. All values are means ± S.E.M (n = 5)Enzyme activities are shown as nmole substrate hydrolyzed by 1 ml effluent for 1 min. All values are means ± S.E.M (n = 5). Lower panels show typical HPLC chromatograms of ATP (B) and AMP (C) hydrolyzed by the first fraction of post-ischemic reperfusate.

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