Fig. 5

Role of UCP2 on the regulation of curcumin on ROS and NO accumulation in HUVECs_. a: SHRsp was fed with curcumin (100 mg/kg/day) for 4 weeks, expression of UCP2 mRNA in carotid artery SHRsp was determined by RT-PCR, Result was indicated as ratio of UCP2 and β-actin. (n = 6). b: HUVECs were exposed to different doses of H₂O₂ (10, 20, 50, 100 μmol) for 24 h. Cell viability was measured by CCK-8 assay (n = 7). c: HUVECs were pre-treated with curcumin (0.01, 0.05, 0.1, 0.5,1, 5 μmol/L) for 2 h and then co-incubated with H₂O₂ (20 μmol/L) for 24 h. The cell viability was measured by CCK-8 assay (n = 7). d-f: HUVECs were exposed to different reagents (H₂O₂ 20 μmol/L, curcumin 1 μmol/L, genipin 10 μmol/L). UCP2 expression was examined by western blot (d). Representative images of ROS (e) and NO (f) staining were shown (D: n = 5; E and F were repeated at least three times)