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Fig. 5 | BMC Cardiovascular Disorders

Fig. 5

From: Mir-195-5p targets Smad7 regulation of the Wnt/β-catenin pathway to promote osteogenic differentiation of vascular smooth muscle cells

Fig. 5

The effects of Smad7 on the osteogenic differentiation of vascular smooth muscle cells (VSMCs) and the Wnt/β-catenin pathway. (A) Cell viability was determined using an MTT assay. (B) Cell apoptosis was determined using flow cytometry. (C–H) The levels of Runt, RUNX2, BMP2, ALP, OCN, and Smad7 mRNAs were determined using RT-qPCR. (I–P) The levels of RUNX2, Smad7, Wnt3a, and β-catenin proteins were determined using western blotting. *p < 0.05 vs. ov-Smad7 group, #p < 0.05 vs. ov-Smad7-NC + KYA1797K group. M, N, O, and P are blots obtained for days 0, 3, 7, and 14 of osteogenic differentiation, respectively. Control group: VSMCs without osteogenic differentiation. Other groups: VSMCs with osteogenic differentiation

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BMC Cardiovascular Disorders

ISSN: 1471-2261

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