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Fig. 4 | BMC Cardiovascular Disorders

Fig. 4

From: Mir-195-5p targets Smad7 regulation of the Wnt/β-catenin pathway to promote osteogenic differentiation of vascular smooth muscle cells

Fig. 4

Dual-luciferase reporter assay and transfection. (A) An alignment of Smad7 mRNA 3ʹUTR with the miR-195-5p seed sequence. (B) Dual-luciferase reporter assay. *p < 0.05 vs. empty vector group, #p < 0.05 vs. Smad7 3′-UTR (Mutant) group. (C) The levels of Smad7 mRNA were determined using RT-qPCR. (D and E) The levels of Smad7 protein were determined using western blotting. *p < 0.05 vs. control group, #p < 0.05 vs. ov-Smad7-NC group

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BMC Cardiovascular Disorders

ISSN: 1471-2261

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