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Fig. 2 | BMC Cardiovascular Disorders

Fig. 2

From: Mir-195-5p targets Smad7 regulation of the Wnt/β-catenin pathway to promote osteogenic differentiation of vascular smooth muscle cells

Fig. 2

The effects of miR-195-5p on the osteogenic differentiation of vascular smooth muscle cells (VSMCs) and expression of Wnt/β-catenin pathway constituents. (A) Levels of miR-195-5p were determined using RT-qPCR. (B) Cell viability was determined using an MTT assay. (C) Cell apoptosis was determined using flow cytometry. (D–I) The levels of Runt, RUNX2, BMP2, ALP, OCN, and Smad7 mRNAs were determined using RT-qPCR. (J–N) The levels of RUNX2, Smad7, Wnt3a, and β-catenin proteins were determined using western blotting. *p < 0.05 vs. control group; #p < 0.05 vs. miR-195-5p-NC group

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BMC Cardiovascular Disorders

ISSN: 1471-2261

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