Fig. 1

Diagram of the experimental grouping. Rabbits in group N were fed only ordinary fodder without intervention. Rabbits in group H were fed only high-cholesterol fodder without intervention. Rabbits in the R3qod, R3qd, and R6qd groups were fed with high-cholesterol fodder and received three cycles of RTLI once every other day, three cycles of RTLI once daily, and six cycles of RTLI once daily, respectively. Each cycle of RTLI included 5 min of limb ischemia followed by 5 min limb reperfusion. Rabbits in the S3qod, S3qd, and S6qd groups were fed with high-cholesterol fodder and received sham ischemia for 30 min once every other day, 30 min once daily, and 60 min once daily, respectively. RTLI, regular transient limb ischemia. n = 10 per group