Fig. 4

Signalling pathways regulating transforming growth factor beta-stimulated clone 22 (TSC-22) gene expression. Cultured neonatal rat ventricular myocytes (NRVMs) were treated with endothelin-1 (ET, 100 nM) for 1, 4, 12 and 24 h. a TSC-22 mRNA levels were measured by RT-PCR (n = 9−12 from three independent experiments) b) and TSC-22 total protein levels were determined by Western blot (n = 4). Open bars represent control and solid bars ET **P <0.01, ***P <0.001 versus control (one-way ANOVA followed by least significance difference post hoc test). TSC-22 mRNA levels in NRVMs exposed to kinase inhibitor (c) PD98059 (10 μM) or d) SB203580 (10 μM), and treated with 100 nM ET for 1 h (n = 8−9, from three independent experiments). **P <0.01, ***P <0.001 versus control (Student’s t-test). TSC-22 e) mRNA levels measured by Northern blot and f) total protein levels analysed by Western blot in left ventricle (LV) of Sprague–Dawley (SD)-rats after adenovirus induced MKK3bE and WT p38alpha overexpression at day 3 (n = 7–10). Open bars represent LacZ and solid bars MKK3bE and WT p38alpha **P <0.01 versus LacZ (Student’s t-test). The results are expressed as mean ± SEM. Representative Western blots are shown