Figure 6

Effect of adenoviral siRNA on HIF-1α protein (A) and cell proliferation (B) in HMEC-1 cells. Adenoviral vectors containing hairpin siRNAs against HIF-1α were created as in the methods. Panel A. HMEC-1 cells were infected with 50–100 PFU/cell adenovirus or 5 μM YC-1, exposed to 5% O₂ for 48 hours then cell lysates made and HIF-1α protein expression assessed by Western blot. Representative bands for HIF-1α are shown. Panel B. HMEC-1 cells were infected with adenovirus or treated with 5 μM YC-1 every 3 days for 7 days and cell proliferation assessed using the CFDA-AM assay as in methods. The data represent the mean +/- SEM of triplicate wells from 3 independent experiments. *** = P < 0.001 using one-way ANOVA with a Neuman-Keuls multiple comparisons post-test.