Figure 7
From: Manipulation of valve composition to elucidate the role of collagen in aortic valve calcification
Characterization of leaflet mineralization indicated greater mineralization of collagen-deficient leaflets compared to undepleted controls. (A) von Kossa staining for mineralization was performed after 6 days of culture; calcium deposits are stained black. Higher magnification images of Day 6 samples are included to better visualize the differences in staining between conditions. (B) Calcium content in collagen-deficient leaflets compared to undepleted leaflets after 6 days of culture. Scale bar = 100 μm. *P < 0.05 compared to undepleted.