Figure 3

Determination of apoptosis in the cardiomyocytes. Panel A, Four hours after LPS treatment (4 mg/kg), 4-mm paraffin- embeded tissue sections were prepared from LV apex and TUNEL staining of the nuclei of cardiomyocytes from control and LPS-treated mice was performed. Panel B, The apoptosis index among the sham-treated mice and mice treated with LPS or LPS plus calpain inhibitors is demonstrated. CI, calpain inhibitor-III; PD, PD150606. * P < 0.05 vs. sham, # P < 0.05 vs. LPS (n = 5).