Figure 3
Expression of CAII, CAIV, and ANP proteins in adult human ventricle. A, Lysates were prepared from hypertrophic/dilated (H/D), or non-hypertrophic/non-dilated (NH/ND) adult human ventricles, from explanted failing hearts. Left panels, failing ventricles with no signs of either hypertrophy or dilation were compared with hypertrophic or dilated failing ventricles. Protein samples (30 μg) were resolved by SDS-PAGE, transferred to PVDF membrane, and probed with anti-CAII, anti-CAIV, anti-ANP, and anti-α-actinin antibodies. Right panels, samples from left (L) and right (R) ventricles were directly compared. Filled arrow indicates position of protein. B, Summary of the protein expression normalized to α-actinin. Values are expressed relative to the non-hypertrophic/non-dilated protein expression; (n = 5). C, Summary of the protein expression normalized to α-actinin. Values are expressed relative to the control right ventricle protein expression; (n = 5). *Indicates statistically significant difference (P < 0.05).