Figure 2

Expression of carbonic anhydrases in hearts. A, Lysates were prepared from adult human ventricular sample isolated from explanted heart (see Table two) (50 μg protein), or freshly isolated adult mouse ventricular myocytes (50 μg protein), or HEK293 cells transfected with empty vector (30 μg protein), or HEK293 cells transfected with human CAII (A), human CAIV B), or human CAXIV (C) cDNA (30 μg protein), cDNA. Samples were resolved by SDS-PAGE, transferred to PVDF membrane, and probed with anti-CAII, CAIV or CAXIV as indicated. Filled arrows indicate position of protein.