Post-COVID-19 illness and associations with sex and gender

Background Post-COVID-19 syndromes have associated with female sex, but the pathophysiological basis is uncertain. Aim There are sex differences in myocardial inflammation identified using cardiac magnetic resonance (CMR) in post-COVID-19 patients, and in patient reported health outcomes following COVID-19 infection. Design This prospective study investigated the time-course of multiorgan injury in survivors of COVID-19 during convalescence. Methods Clinical information, blood biomarkers, and patient reported outcome measures were prospectively acquired at enrolment (visit 1) and 28–60 days post-discharge (visit 2). Chest computed tomography (CT) and CMR were performed at visit 2. Follow-up was carried out for serious adverse events, including death and rehospitalization. Results Sixty-nine (43%) of 159 patients recruited were female. During the index admission, females had a lower peak C-reactive protein (74 mg/l (21,163) versus 123 mg/l (70, 192) p = 0.008) and peak ferritin (229 μg/l (103, 551) versus 514 μg/l (228, 1122) p < 0.001). Using the Modified Lake-Louise criteria, females were more likely to have definite evidence of myocardial inflammation (54% (37/68) versus 33% (30/90) p = 0.003). At enrolment and 28–60 days post-discharge, enhanced illness perception, higher levels of anxiety and depression and lower predicted maximal oxygen utilization occurred more commonly in women. The mean (SD, range) duration of follow-up after hospital discharge was 450 (88) days (range 290, 627 days). Compared to men, women had lower rates of cardiovascular hospitalization (0% versus 8% (7/90); p = 0.018). Conclusions Women demonstrated worse patient reported outcome measures at index admission and 28–60 days follow-up though cardiovascular hospitalization was lower.


Introduction
Immune response to infection is sex-specific and likely to drive infection severity risk and mortality risk [1,2].This has been reported for the severe acute respiratory syndrome coronavirus-1 (SARS-CoV1) epidemic [3] and the middle eastern respiratory syndrome (MERS) outbreak [4].Recently, a similar male bias towards severe disease has been reported with SARS-CoV-2 [5][6][7][8] despite similar infection rates between sexes [9].Registry data reports that this mortality difference is only partly due to sex and gender differences in high risk characteristics [7,8] and is independent of age [10].Men were also more likely to have symptomatic COVID-19 illness compared with women [11].
Despite the higher morbidity and mortality in men with acute SARS-CoV-2 infection, a larger percentage of women tend to be at greater risk for long-term COVID-19 manifestations irrespective of baseline severity of disease [12,13].In fact, women were less likely to report a recovery back to baseline, and seven times as likely to report dyspnea, and twice as likely to report fatigue [14].
Prior studies using cardiovascular magnetic resonance imaging in COVID-19 have reported imaging features of myocardial inflammation in 27-60%, [15][16][17][18] of patients but the presence and nature of sex associations are incompletely understood.
Our hypotheses were that there are sex differences in myocardial inflammation identified using cardiac magnetic resonance (CMR) in post-COVID-19 patients scanned at 28-60 days, and in patient reported health outcomes following COVID-19 infection.

Design
The design and the main findings of the CISCO-19 study have been reported [19,20].In this manuscript the terms male and female have been used when referring to biological sex, whereas man and woman are used when referring to psychosocial gender, or when these factors are not clear.
In summary, this study involved a prospective, observational, longitudinal, cohort design to assess the timecourse of multiorgan injury in survivors of COVID-19 during convalescence [19].Clinical information, a 12-lead digital ECG, blood and urine biomarkers, and patient reported outcome measures were acquired at enrolment (visit 1) and again during convalescence, 28-60 days post-discharge (visit 2).Chest computed tomography (CT), including pulmonary and coronary angiography, and cardiac MRI were carried out at visit 2. This sexbased analysis of the primary and secondary outcomes was prespecified.

Participant identification
Patients who received hospital care for COVID-19, with or without admission, and were alive, were prospectively screened in real time using an electronic healthcare information system (TrakCare ® , InterSystems ® , USA) and daily hospital reports identifying inpatients with laboratory-positive results for COVID-19.

Eligibility criteria
The inclusion criteria were: (1) age ≥ 18 years old; (2) history of an unplanned hospital visit e.g., emergency department, or hospitalization > 24 h for COVID-19 confirmed by a polymerase chain reaction (PCR) test; (3) ability to comply with study procedures; and (4) ability to provide written informed consent.Imaging results were reported by accredited radiologists according to contemporary, national guidelines [21].

Research schedule
The protocol involved two visits.The first visit involved informed consent and assessments during the initial hospitalization, or as soon as possible after discharge.The second visit occurred 28-60 days post-discharge.This window was positioned to reflect the convalescent phase and give sufficient scope to schedule the patients.
The procedures involved prospective collection of clinical data and a time-course of research investigations.Clinical data included demographics, medical and cardiovascular history, findings from clinical examinations, laboratory and radiological tests, cardiology tests (including an electrocardiogram (ECG) and an echocardiogram if available) and treatment.The research investigations at both visits included blood and urine samples, a 12-lead ECG, health status questionnaires, and assessments of adverse events.Heart, lung, and kidney imaging were acquired at the second visit.

Multimodality imaging CT
A 320-detector CT scanner (Aquilion ONE, Canon Medical Systems Corp.) provided full heart coverage within a single heartbeat.Non-contrast and contrastenhanced angiographic breath-hold ECG-gated volumes were acquired and timed for optimum pulmonary and systemic arterial (coronary) opacification.Patients with severe renal dysfunction underwent non-contrast CT.

Cardiovascular MRI
Cardiovascular MRI was undertaken to measure heart structure and function and assess for persisting evidence of myocardial injury and/or myocardial infarction using multi-parametric techniques.All patients underwent protocol-directed MRI in the convalescent phase, 28-60 days after discharge.The scan protocol included cine-imaging of cardiac anatomy and function and myocardial tissue characterization using multiparametric techniques.They included 1) mapping myocardial native longitudinal relaxation time (T1 in milliseconds) using the modified Look-Locker inversion recovery technique (T1-mapping) before and after intravenous administration of gadolinium contrast media (0.15 mmol/ kg of Magnevist, Bayer Healthcare), 2) mapping transverse relaxation time (T2 in milliseconds), 3) first pass contrast-enhanced perfusion and 4) late gadoliniumenhancement imaging.
The expert consensus recommendations for the MRI diagnostic criteria of non-ischemic myocardial inflammation (modified Lake Louise criteria) were used to diagnose definite myocardial inflammation (abnormal T2 and T1 (native T1, late gadolinium enhancement or extracellular volume)) or probable myocardial inflammation (abnormal: T2 or T1) [24] To limit selection bias, patients with severe renal dysfunction (GFR < 45 ml/kg/ m 2 ) were not excluded but did not have gadolinium contrast.Quantitative analyses were undertaken in a central laboratory, blinded to the other clinical data.

Health status and patient reported outcome measures
Questionnaires were completed by participants at visit 1 (enrolment) and visit 2 (28-60 days after the last episode of hospital care).Self-reported health status was assessed using EuroQOL EQ-5D-5L and the Brief Illness Perception Questionnaire (Brief-IPQ).The Patient Health Questionnaire-4 (PHQ-4) was utilized to assess for anxiety and depressive disorders.The Duke Activity Status Index (DASI) was used to assess predicted maximal oxygen utilization.The International Physical Activity Questionnaire-Short Form (IPAQ-SF) measures the forms and intensity of physical activity and sitting time that participants do as part of their daily lives.

Longitudinal follow-up for clinical outcomes
Clinical research team members assessed study participants' electronic health records.Serious adverse events (SAE), (comprising death and rehospitalization), outpatient clinic visits were assessed as part of follow-up.
Cardiovascular and respiratory SAE were independently reviewed and adjudicated by the clinical event committee team members.

Statistics
Statistical analysis was carried out by the Robertson Centre for Biostatistics.Summary statistics are presented as Mean ± SD, Median (IQR), or N (%).Normally distributed continuous variables, non-normally distributed continuous variables, and categorical variables were analysed by T-Test, Wilcoxon-Mann-Whitney Test, or Fisher's Exact Test respectively.
Linear mixed effects models for patient reported outcome measures (PROMs) include random effects for subjects, fixed effects for age and sex, fixed effects for study visits, and an interaction between sex and visit.Model-derived estimates are reported for differences between women and males at each study visit, and for mean changes between study visits in women and men, with 95% confidence intervals and two-sided p-values.P-values for sex-by-visit interactions are also reported.Duration of follow-up is summarised as Median (IQR), and compared between groups using a Wilcoxon-Mann-Whitney test.Clinical outcomes by sex are summarised, and compared between groups using log rank tests of the time to first event.No adjustments were made for multiplicity, and a p-value of less than 0.05 was considered statistically significant.

Trial management and timelines
The study was conducted in line with the current Guidelines for Good Clinical Practice in Clinical Trials and STrengthening the Reporting of OBservational studies in Epidemiology guidelines, and coordinated by a Study Management Group.A Scientific Steering Group had oversight of the study.

Ethics
The study was approved by the UK National Research Ethics Service (Reference 20/NS/0066).

Results
One thousand three hundred and six patients were screened between 22 May 2020 and 16 March 2021 and 267 patients provided written informed consent (Cognitive impairment, n = 87; frailty, n = 341; death, n = 101; no consent, n = 356; non-compliance with protocol, n = 154).

Electrocardiology
There were no differences in ECG criteria by sex (Table 2).

Health status
Compared to men, at enrolment and 28-60 days postdischarge, women demonstrated enhanced illness perception, higher levels of anxiety and depression and lower predicted maximal oxygen utilization (ml/kg/min) (Table 3).Furthermore, at 28-60 days post-discharge women had lower health-related quality of life, and lower levels of physical activity (Table 3).
Adjusting for age and study visits, health-related quality of life, enhanced illness perception, higher levels of anxiety and depression, lower levels of physical activity and lower predicted maximal oxygen utilization were evident in women (Table 4).

Serious adverse events
Follow-up was continued to December 13, 2021 for all participants.The mean (SD, range) duration of followup after hospital discharge was 450 (88) days (range 290, 627 days).

Discussion
This study assessed sex and gender differences in a deeply phenotyped cohort of patients utilizing serum and urine biochemistry, patient reported outcomes and electrocardiograms at baseline and 28-60 days after hospital discharge; cross sectional imaging with computed tomography and magnetic resonance imaging and clinical follow-up up to a mean of 450 days after hospital discharge.
The main findings were: 1.No difference in COVID-19 illness severity between women and men with regards to length of stay or therapy (including intensive care).2. Male sex was associated with history of hypertension, myocardial infarction and heart failure  was associated with higher rates of definitive myocardial inflammation utilizing the Modified Lake-Louise criteria.7. Worse patient reported outcome measures at index admission and 28-60 days follow-up were demonstrated in women compared to men. 8. Female sex was associated with lower rates of hospitalization for cardiovascular disease in the longer term after COVID-19 infection.
Unlike the meta-analysis reported by Peckham et al. [9], where men had higher odds of requiring intensive care admission or death, we did not identify a difference in acute disease severity for women and men in our smaller study.However, we report persisting changes identified on magnetic resonance imaging and patient reported outcome measures associated with female sex.These findings complement data reported by Bai et al. [12] Sylvester et al. [13] and others that women tend to be at higher risk for COVID-19 manifestations persisting in the longer term irrespective of baseline severity of disease [12,13].
Women had lower rates of hypertension, myocardial infarction or heart failure, and no difference in other past medical history parameters commonly reported as being associated with worse outcomes in COVID-19 infection including diabetes or smoking status [26].There was no sex difference in various pulmonary computed tomographic parameters including consolidation, reticulation, atelectasis or thrombosis.A retrospective study looking at 1165 patients reported that female sex was associated with less severe lung involvement [27].These differences could be due to study design (prospective versus retrospective) and different inclusion criteria (all hospitalized patients) and data analyses (lung involvement quantified utilizing an AI system [28]).
In this study, female sex was associated with lower peak markers of inflammation when compared with male sex, consistent with data reported from the MGH COVID-19 patient registry (n = 781) [29].These observations point to an immunological basis in the different course of the disease according to sex as per Brodin [30].
To date, there are no reports describing sex-and/or gender-specific differences in myocardial injury utilizing cardiac magnetic resonance imaging in COVID-19 patients in the acute or longer-term [15][16][17][18].Whilst elevated T1 values per se may be a non-specific finding on its own (elevated in patients with hypertensive heart disease, diabetes), when analyzed as part of the modified Lake-Louise criteria [24] i.e. having both a Summaries are Mean ± SD, Median (IQR), or N (%).P-values from T-Test, Wilcoxon-Mann-Whitney Test, or Fisher's Exact Test A number of studies looking at both hospitalized and non-hospitalized patients [13,[31][32][33] with COVID-19 reported higher rates of depression, anxiety and illness perception in women participants.There were similar rates of outpatient healthcare uptake between men and women, and similar rates of serious adverse events (death or rehospitalization).In our study, female sex was associated with lower rates of hospitalization for cardiovascular disease in the longer term after COVID-19 infection.
The apparent discrepancy between higher rates of patient reported outcomes in women and increased hospitalization for cardiovascular causes in men could be explained by the small sample size in our study (possible type 1 error), the higher, pre-exisiting cardiovascular disease burden in men, or the possibility that the hospitalization events for cardiovascular causes were not related to COVID-19 infection.
Imaging was carried out from day 28 post discharge to align with the International Severe Acute Respiratory and Emerging Infection Coronavirus Clinical Characterisation Consortium (ISARIC4C) study [34].Most of the study participants were unvaccinated • thorough peer review by experienced researchers in your field • rapid publication on acceptance • support for research data, including large and complex data types • gold Open Access which fosters wider collaboration and increased citations maximum visibility for your research: over 100M website views per year

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Table 1
Clinical characteristics of the study population, by sex

Table 2
Multisystem phenotypying by sex: serial electrocardiography, biomarkers of inflammation, metabolism, renal function, and haemostasis, and heart, lung, and kidney imaging at 28-60 days post-discharge

Table 3
Health status, illness perception, anxiety and depression, and physical function by sexSummaries are Mean ± SD, Median (IQR), or N (%).P-values from T-Test, Wilcoxon-Mann-Whitney Test, or Fisher's Exact Test