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Table 1 Polymerase chain reaction process

From: Association between AT1 receptor gene polymorphism and left ventricular hypertrophy and arterial stiffness in essential hypertension patients: a prospective cohort study

Protocol

Temperature (°C)

Time (s)

 

Pre-denaturation

95

30

30 cycles

Denaturation

95

10

Annealing

60

30

Extension

70

30