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Fig. 4 | BMC Cardiovascular Disorders

Fig. 4

From: Vitamin C alleviates LPS-induced myocardial injury by inhibiting pyroptosis via the ROS-AKT/mTOR signalling pathway

Fig. 4

Vitamin C protects H9C2 cells against LPS-induced cell death through the ROS-AKT/mTOR-pyroptosis pathway. A Intracellular ROS level detected by DCFH-DA (magnification 200 ×). B Nox4 expression detected by western blotting. The cropped blots are shown on the left. The relative protein expression levels compared with the β-actin levels are shown on the right. Full-length blots are presented in Additional file 1: Figure S3A. C The expression of pyroptosis-associated proteins and p-AKT and p-mTOR was detected by western blotting. The cropped blots are shown on the left. The relative protein expression levels compared with the β-actin levels are shown on the right. 1: control (CTL) group, 2: LPS group, 3: LPS + NAC group, 4: LPS + Vc group. Full-length blots are presented in Additional file 1: Figure S3B. The full length membrane with edges visible of p-AKT was not provided because that the rest of the membrane belongs to a different experiment. D Levels of IL-1β and IL-18 in the culture supernatants. Data are presented as mean ± SD (n = 3). E Cell viability assessment by CCK-8 assay. Data are presented as mean ± SD (n = 5). F LDH release assays. Data are presented as mean ± SD (n = 3). G CK-MB release assays. Data are presented as mean ± SD (n = 3). #p < 0.05 compared with the LPS group; ##p < 0.01 compared with the LPS group; ###p < 0.001 compared with the LPS group

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