Fig. 3

LPS induces pyroptosis through the AKT/mTOR pathway in H9C2 cells. A H9C2 cells were treated with the indicated concentrations of LPS, and the p-AKT and p-mTOR protein levels were detected by western blotting. The cropped blots are shown on the left. The relative protein expression levels compared with the β-actin levels are shown on the right. Full-length blots are presented in Additional file 1: Figure S2A. B Pyroptosis-associated proteins detected by western blotting. The cropped blots are shown on the left. The relative protein expression levels compared with the β-actin levels are shown on the right. 1: control (CTL) group, 2: LPS group, 3: LPS + MK-2206 group. Full-length blots are presented in Additional file 1: Figure S2B. C Levels of IL-1β and IL-18 in the culture supernatants. Data are presented as mean ± SD (n = 3). D Cell viability assessment by CCK-8 assays. Data are presented as mean ± SD (n = 5). E LDH release assays. Data are presented as mean ± SD (n = 3). F CK-MB release assays. Data are presented as mean ± SD (n = 3). *p < 0.05 compared with the control (CTL) group; **p < 0.01 compared with the CTL group; ns, p > 0.05 compared with the CTL group. #p < 0.05 compared with the LPS group; ##p < 0.01 compared with the LPS group; ###p < 0.001 compared with the LPS group