Fig. 4

Alteration of PI3K/Akt signalling pathway in diabetic rats after I-Post or nicorandil treatment alone A Representative western blot showing total expression and the phosphorylation state. Comparison of p-PI3K expression levels in myocardial tissue of rats treated with I-Post or nicorandil (n ≥ 3). B Comparison of p-Akt expression levels in myocardial tissue of rats treated with I-Post or nicorandil (n ≥ 3). C Comparison of p-GSK3βexpression levels in myocardial tissue of rats treated with I-Post or nicorandil (n ≥ 3). D Comparison of p-mTOR expression levels in myocardial tissue of rats treated with I-Post or nicorandil (n ≥ 3). E Comparison of p-eNOS expression levels in myocardial tissue of rats treated with I-Post or nicorandil (n ≥ 3). F NO plasma levels in rats treated with I-Post or nicorandil (n = 6). Compared with I/R: ^†††P < 0.001; Compared with DM I/R: ^‡‡‡P < 0.001; Comparison between nondiabetic and diabetic groups applying the same treatment: ***P < 0.001, **P < 0.01, *P < 0.05, ^nsP > 0.05. Data represent the mean ± SEM. Data were analysed using one-way ANOVA and Bonferroni multiple comparisons test. Sham, sham surgery; I/R, ischaemia‒reperfusion; I-Post, ischaemia postconditioning, Nic, nicorandil; DM, diabetes mellitus