Fig. 5
ROCK2 was regulated by miR-942-5p. A The binding sites of miR-942-5p and ROCK2 were displayed. B–D The relationship between ROCK2 and miR-942-5p was assessed by dual-luciferase reporter assay, RIP assay, and RNA pull-down assay. E ROCK2 level was assessed in serum samples from 21 patients with atherosclerosis and 17 healthy volunteers using qRT-PCR. F The expression correlation between ROCK2 and miR-942-5p in 21 atherosclerosis tissues was analyzed by Pearson correlation analysis. G The protein expression of ROCK2 in ox-LDL-treated HUVECs was determined using western blot assay. H qRT-PCR analysis of miR-942-5p in HUVECs treated with Control, ox-LDL, ox-LDL + anti-miR-NC, or ox-LDL + anti-miR-942-5p. I and J ROCK2 protein expression was assessed in HUVECs treated with Control, ox-LDL, ox-LDL + miR-NC, ox-LDL + miR-942-5p, ox-LDL + anti-miR-NC, or ox-LDL + anti-miR-942-5p using western blot assay. **P < 0.01; ***P < 0.001; ****P < 0.0001. All tissue and cellular experiments were independently repeated three times. Student’s t-test was used to analyze the differences in (D, E), one-way ANOVA was utilized to assess the differences in (G, H, and I), and two-way ANOVA was utilized to analyze the differences in (B, C)