Fig. 3
Circ_0004104 acted as a sponge of miR-942-5p. A The binding sites between circ_0004104 and miR-942-5p were shown. B MiR-942-5p expression in HUVECs transfected with miR-942-5p mimics using qRT-PCR. C The luciferase activities of WT-circ_0004104 and MUT-circ_0004104. D RIP assay was performed to analyze the enrichment of circ_0004104 and miR-942-5p. E Their interaction was verified using RNA pull-down assay. F qRT-PCR analysis of miR-942-5p in serum samples from 21 patients with atherosclerosis and 17 healthy volunteers. G Pearson correlation analysis was applied to evaluate the expression association between circ_0004104 and miR-942-5p in 21 atherosclerosis patients. H qRT-PCR analysis of miR-942-5p in HUVECs treated with 0 μg/mL, 25 μg/mL, 50 μg/mL and 100 μg/mL of ox-LDL. I qRT-PCR analysis of circ_0004104 in HUVECs treated with Control, ox-LDL, ox-LDL + pCD5-ciR, and ox-LDL + circ_0004104. J qRT-PCR analysis of miR-942-5p in HUVECs treated with Control, ox-LDL, ox-LDL + si-NC, ox-LDL + si-circ_0004104, ox-LDL + pCD5-ciR, or ox-LDL + circ_0004104. **P < 0.01; ***P < 0.001; ****P < 0.0001. All tissue and cellular experiments were independently repeated three times. Student’s t-test was used to analyze the differences in (B, E, and F), one-way ANOVA was utilized to assess the differences in (H, I, and J), and two-way ANOVA was utilized to analyze the differences in (C, D)