Table 1 Primer sequences for PCR and amplicon lengths and SNaPhot primers were used in this study
SNP | Forward Primer (5′–3′) | Reverse Primer(5′–3′) | Size (bp) | Tm (°C) |
|---|---|---|---|---|
PCR primers | ||||
IL-1α | GCTTCACTATGTTGCCCACA | CAGTAAAGTAGCCCTCTACCAA | 462 | 58 |
IL-1β | GGCATTGATCTGGTTCATCC | GTTTAGGAATCTTCCCACTT | 304 | 56 |
TNFα | GAAACAGACCACAGACCTGG | ACACAAGCATCAAGGATACC | 177 | 58 |
IL-6 | CACGAAATTTGAGGATGGCC | GGCTGATTGGAAACCTTATT | 489 | 56 |
SNaPshot primers | ||||
IL-1α, rs1800587 | ATAATAGTAACCAGGCAACA | TTACATATGAGCCTTCAATG | ||
IL-1β, rs16944 | TGCAATTGACAGAGAGCTCC | TGGGTGCTGTTCTCTGCCTC | ||
TNFα, rs1800629 | AATAGGTTTTGAGGGGCATG | CTGGAGGCTGAACCCCGTCC | ||
TNFα, rs361525 | AGAAGACCCCCCTCGGAATC | ACTCCCCATCCTCCCTGCTC | ||
IL-6, rs1800795 | TCCCCCTAGTTGTGTCTTGC | AATGTGACGTCCTTTAGCAT | ||
IL-6, rs1800796 | CAGGCAGTTCTACAACAGCC | GTTCTGGCTCTCCCTGTGAG | ||