Fig. 5

Silencing of BDNF partially reverses the effect of sh-G9a on alleviating cardiomyocyte apoptosis and injury by inhibiting the TrkB signaling pathway. A Detection of extent of TrkB phosphorylation in H9C2 cells transfected with sh-G9a by Western blot (Uncropped protein blots are provided in the Additional file 1). B The protein levels of BDNF, TrkB and p-TrkB in cells transfected with sh-G9a + sh-BDNF expression by Western blot (Uncropped protein blots are provided in the Additional file 1). C The proliferative activity of cells measured by CCK8 assay. D The apoptotic activity of cells measured using flow cytometry. E Detection of cytotoxicity of cells by LDH cytotoxicity kits. F The levels of fibrosis-related factors α-SMA and FN1 in cells examined using Western blot (Uncropped protein blots are provided in the Additional file 1). Data are expressed as mean ± SD. Each experiment was repeated at least three times. Three or more groups were compared by one-way or two-way ANOVA and Tukey's post hoc test. *p < 0.05, **p < 0.01