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Table 3 Final rankings of the candidate reference genes

From: Normalization strategy for selection of reference genes for RT-qPCR analysis in left ventricles of failing human hearts

Final ranking

Distribution of diseased samples

Healthy vs. failing

Random groups of 5

Individual cardiomyopathies

1

IPO8

IPO8

IPO8

2

POLR2A

POLR2A

POLR2A

3

PGK1

UBC

GAPDH

4

UBC

GAPDH

HPRT1

5

HPRT1

PGK1

UBC

6

GAPDH

PPIA

PGK1

7

PPIA

HPRT1

PPIA

8

GUSB

TBP

GUSB

9

HMBS

HMBS

HMBS

10

TFRC

GUSB

TRFC

11

-

TRFC

-

  1. The final raking was calculated as a geometric mean of ranking positions from (ANOVA, geNorm, NormFinder, BestKeeper and Delta Cq method), the two most stably expressed genes are shown in bold