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Table 3 Final rankings of the candidate reference genes

From: Normalization strategy for selection of reference genes for RT-qPCR analysis in left ventricles of failing human hearts

Final ranking Distribution of diseased samples
Healthy vs. failing Random groups of 5 Individual cardiomyopathies
1 IPO8 IPO8 IPO8
2 POLR2A POLR2A POLR2A
3 PGK1 UBC GAPDH
4 UBC GAPDH HPRT1
5 HPRT1 PGK1 UBC
6 GAPDH PPIA PGK1
7 PPIA HPRT1 PPIA
8 GUSB TBP GUSB
9 HMBS HMBS HMBS
10 TFRC GUSB TRFC
11 - TRFC -
  1. The final raking was calculated as a geometric mean of ranking positions from (ANOVA, geNorm, NormFinder, BestKeeper and Delta Cq method), the two most stably expressed genes are shown in bold