Skip to main content

Table 2 Stability values and significance levels of the CRGs

From: Normalization strategy for selection of reference genes for RT-qPCR analysis in left ventricles of failing human hearts

Gene Variance component F Significance VB VW Stability index Ranking
Between groups Within group
IPO8 0.002 0.061 0.037 0.810 0.004 0.107 0.00042 1
PGK1 0.013 0.054 0.232 0.538 0.036 0.155 0.006 2
HPRT1 0.104 0.053 1.957 0.358 0.203 0.104 0.021 3
GAPDH 0.134 0.059 2.276 0.089 0.243 0.107 0.026 4
YWHAZ 0.623 0.041 15.254 0.036 0.775 0.051 0.039 5
POLR2A 0.225 0.057 3.928 0.115 0.408 0.104 0.042 6
PPIA 0.275 0.143 1.920 0.255 0.339 0.177 0.060 7
TBP 0.244 0.031 7.883 0.007 0.695 0.088 0.061 8
TRFC 0.448 0.212 2.114 0.374 0.364 0.172 0.063 9
UBC 0.208 0.091 2.283 0.212 0.386 0.169 0.065 10
HMBS 0.735 0.213 3.450 0.147 0.654 0.190 0.124 11
GUSB 0.472 0.121 3.908 0.051 0.757 0.194 0.147 12
RPLP0 2.356 0.123 19.167 0.021 1.959 0.102 0.200 13
B2M 2.487 0.093 26.871 0.0001 2.499 0.093 0.232 14
ACTB 3.278 0.114 28.636 0.007 2.637 0.092 0.243 15
  1. P-values were calculated using Welch’s ANOVA test. In bold, p < 0.05