Table 2 Kit and antibody list
Kit or antibody | Supplier | Cat# | Dilution |
|---|---|---|---|
ClonExpress Ultra One Step Cloning Kit | Vazyme | C115-01 | NA |
Rat IL-1β/IL-1F2 Immunoassay | R&D | RLB00 | NA |
Rat IL-6 Immunoassay | R&D | R6000B | NA |
Rat IL-10 Immunoassay | R&D | R1000 | NA |
Rat CCL2/JE/MCP-1 Immunoassay | R&D | DY3144-05 | NA |
Rat TNF-α Immunoassay | R&D | RTA00 | NA |
GAPDH | Proteintech | 10494-1-AP | 1:3000 |
MG53 | Proteintech | 22151-1-AP | 1:5000 |
Bcl-2 | Abcam | ab194583 | 1:1000 |
Bax | Abcam | ab232479 | 1:1000 |
Cleaved caspase 3 | Cell Signaling Technology | 9661 | 1:1000 |
Cleaved caspase 9 | Cell Signaling Technology | 9507 | 1:1000 |
Cyclin D1 | Abcam | ab134175 | 1:1000 |
Cyclin A1 | Abcam | ab53699 | 1:1000 |
Cyclin E1 | Proteintech | 11554-1-AP | 1:1000 |
CDK2 | Proteintech | 10122-1-AP | 1:2000 |
P65 | Proteintech | 66535-1-Ig | 1:3000 |
P-P65 | Abcam | ab76302 | 1:1000 |
IkBa | Proteintech | 10268-1-AP | 1:2000 |
p- IkBa | Cell Signaling Technology | 9246 | 1:1000 |
HRP Goat Anti-Rabbit IgG(H + L) HRP Goat Anti-Mouse IgG(H + L) | Proteintech Proteintech | SA00001-2 SA00001-1 | 1:10,000 |
1:10,000 |