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Fig. 2 | BMC Cardiovascular Disorders

Fig. 2

From: Rosuvastatin exerts cardioprotective effect in lipopolysaccharide-mediated injury of cardiomyocytes in an MG53-dependent manner

Fig. 2

Effect of LPS on cell viability in H9C2 cells with or without RS. A Cell viability was analyzed and calculated as percentage. H9C2 cells were treated with 10 μg/ml LPS and different concentrations of RS for 24 h. Data are represented as mean ± SD of six biological replicates. RS: Rosuvastatin. B Mitochondrial membrane potential was determined using JC-1 assay. Representative images from three independent experiments. C Quantitation of mitochondrial membrane potential level indicated using MFI. D Cell apoptosis was analyzed using Annexin-V/PI staining via FACS. Representative images from three independent experiments. E Quantitation of apoptotic cells. F Quantitation of mRNA expression of cytokines, namely IL-1β, IL-6, TNF-a, and MCP-1, as estimated by RT-qPCR. Each experiment was performed in triple. G Quantitation of the level of cytokines, namely IL-1β, IL-6, TNF-a, and MCP-1, as determined using ELISA Data are represented as mean ± SD of three biological replicates. H9C2 cells were treated with 10 μg/mL LPS and 1.0 μM RS for 24 h in (B–G). Group differences were analyzed using one-way ANOVA with the Tukey’s post-hoc test. ns, not significant; **P < 0.01; ***P < 0.005; ****P < 0.001

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