Fig. 5
From: A20 (TNFAIP3) alleviates viral myocarditis through ADAR1/miR-1a-3p-dependent regulation
miR-1a-3p promotes inflammation and apoptosis of CVB3-infected cardiomyocytes by targeting A20. Notes CVB3-infected primary neonatal rat cardiomyocytes and H9c2 cells were transfected with miR-1a-3p inhibitor, inhibitor NC, miR-1a-3p inhibitor + LV-sh-NC or miR-1a-3p inhibitor + LV-sh-A20. A qRT-PCR detected the expression of miR-1a-3p; qRT-PCR (B) and western blotting (C) detected the expression of A20; ELISA measured the levels of IL-6 (D), IL-18 (E) and TNF-α (F) in the supernatant; G flow cytometry assessed the cell apoptosis. *P < 0.05; **P < 0.01; ***P < 0.001; CVB3 Coxsackie virus B3