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Fig. 6 | BMC Cardiovascular Disorders

Fig. 6

From: Proteome-scale profiling reveals MAFF and MAFG as two novel key transcription factors involved in palmitic acid-induced umbilical vein endothelial cell apoptosis

Fig. 6

Alleviation of early apoptosis induced by PA in SV40T-transformed HUVECs overexpressing MAFF and MAFG. a RT-qPCR validation of MAFF OE. b RT-qPCR validation of MAFG OE. c WB validation of MAFF OE. d WB validation of MAFG OE. e–h The first apoptosis assay by flow cytometry. il The second apoptosis assay by flow cytometry. mp the third time of apoptosis assay by flow cytometry. e, i, m Control-500 μM PA group subjected to three independent apoptosis assays by flow cytometry. f, j, n Plasmid-500 μM PA group subjected to three independent apoptosis assays by flow cytometry. g, k, o Results for the MAFF OE-500 μM PA group in the three independent apoptosis assays by flow cytometry. h, l, p Results for the MAFG OE-500 μM PA group in the three independent apoptosis assays by flow cytometry. q Histogram summary results in the four groups. The dots located in Q4 represent early apoptotic cells. OE represents overexpression. ** indicates 0.001 < P < 0.01 and *** indicates P < 0.001. Control-500 μM PA: without transfection and with 500 μM PA stimulation. Plasmid-500 μM PA: with empty plasmid transfection and with 500 μM PA stimulation. MAFF OE-500 μM PA: MAFF OE plasmid transfection and 500 μM PA stimulation. MAFG OE-500 μM PA: MAFG OE plasmid transfection and 500 μM PA stimulation

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