Table 1 Primers used for quantitative real time PCR analysis
Gene | Primer sequence 5’-3’ |
|---|---|
Hsa-miR-33 | F: ACACTCCAGCTGGGGTGCATTGTAGTTGCAT RT: CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGTGCAATG |
All | R: CTCAACTGGTGTCGTGGA |
ABCA1 | F: ACCCACCCTATGAACAACATGA R: GAGTCGGGTAACGGAAACAGG |
CS | F: AACTGCTACCCAAGGCTAAGG R: CTTTTGAGAGCCAAGATACCTGT |
HMGCR | F: AACTGCTACCCAAGGCTAAGG R: CTAAAATTGCCATTCCACGAGC |
U6 | F: CTCGCTTCGGCAGCACA R: AACGCTTCACGAATTTGCGT |
GAPDH | F: TGTTCGTCATGGGTGTGAAC R: ATGGCATGGACTGTGGTCAT |