Table 2 The double-stranded biotinylated oligonucleotides for EMSA
From: Identification and functional study of GATA4 gene regulatory variants in atrial septal defects
Regulatory variants | Oligonucleotide sequences | Locations |
|---|---|---|
g.31360 T>C (rs372004083) | 5′-TTTTTACACGGTAA(T/C)AGGGGCCCTGTGATTG-3′ | 31346–31375 |
g.31436G>A | 5′-CCCGCTGCGCTGGG(G/A)CCTCCAGGCTCTGAC-3′ | 31422–31451 |
g.31437C>A (rs769262495) | 5′-CCGCTGCGCTGGGG(C/A)CTCCAGGCTCTGACG-3′ | 31423–31452 |
g.31487C>G | 5′-GACACATTCCCCTC(C/G)CCCATACCCTGGAAG-3′ | 31473–31502 |
g.31856C>T | 5′-AACTAGCATCCAGC(C/T)GGGCACCCCGGGTGA-3′ | 31842–31871 |