Fig. 6

mmu_circ_0005019 functions as a sponge for miR-499-5p. A Schematic of mmu_circ_0005019-WT (wild-type) and mmu_circ_0005019-MUT (mutant) pmirGLO Dual-Luciferase luciferase vectors. B Luciferase assays of cardiomyocytes cells co-transfected with mmu_circ_0005019-WT or mmu_circ_0005019-MUT reporter and miR-499-5p mimics or negative control (NC) mimics. n = 3. *Student’s t-test, P < 0.05; # Student’s t-test, P > 0.05. C mmu_circ_0005019 and miR-499-5p levels were determined by qRT-PCR after Ago2 or IgG RIP assay. n = 3. *Student’s t-test or Mann–Whitney U test, P < 0.05. D Relative expression of Kcnn3 of cardiomyocytes after co-transfection with mmu_circ_0005019 or control vector and miR-499-5p mimics or NC mimics. n = 3. * Student’s t-test, P < 0.05. The results are expressed as the mean ± sem