Fig. 4
Effect of mmu_circ_0005019 knockdown on the apoptosis and expression of transient outward potassium channel (Ito), voltage-gated sodium channel (INA) and small conductance calcium-activated potassium channel current 3 (SK3) in cardiomyocytes. A Apoptosis of cardiomyocytes transfection with mmu_circ_0005019 siRNAs or negative control si-NC were determined by Annexin V-FITC staining flow cytometry (Student’s t-test). n = 3. B Relative expression of Kcnd1, Kcnd3, Scn5a and Kcnn3 of cardiomyocytes transfection with mmu_circ_0005019 siRNAs or negative control si-NC were determined by qRT-PCR (Student’s t-test and Mann–Whitney U test). For Kcnd1 after si-1 transfection, n = 6; for Kcnd3 and Scn5a after si-1 transfection, n = 4; for the others, n = 3. The results are expressed as the mean ± sem. *si-1 or si-3 vs. si-NC, P < 0.05